Molecular Neuroprotection in stroke

Based on expression profiling data, molecular mechanisms of neuroprotection were analysed using molecular techniques (PCR, serial analysis of gene expression, microarrays, PCR, Northern blotting, etc.) in combination with specific animal models (knockout mice, transgenic mice, etc.). Besides improvement of molecular techniques (Kannbley et al. 2003), specific genes identified by molecular means were analysed with respect to their functional role in cerebral ischemia (e.g. Trendelenburg et al. 2002) and with a current stress on the complement system.

Methods

Selected references

Ziegler G, Harhausen D, Schepers C, Hoffmann O, Rohr C, Prinz V, Konig J, Lehrach H, Nietfeld W, Trendelenburg G (2007)
TLR2 has a detrimental role in mouse transient focal cerebral ischemia. Biochem Biophys Res Commun 359:574-9.

Trendelenburg G, Dirnagl U (2005)
Neuroprotective role of astrocytes in cerebral ischemia: Focus on ischemic preconditioning. Glia. 50:307-20

Kannbley U, Kapinya K, Dirnagl U, Trendelenburg G (2003).
Improved protocol for SAGE tag-to-gene allocation. Biotechniques 34:1212-9

Trendelenburg G, Prass K, Priller J, Kapinya K, et al. (2002).
Serial analysis of gene expression identifies metallothionein-II as major neuroprotective gene in mouse focal cerebral ischemia. J Neurosci 22:5879-88.