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    Campus Charité MitteCharitéplatz 1 (local address: Bonhoefferweg 3)
    10117 Berlin

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Programm

General Information:  

The course will be held in English.

We strongly recommend to prepare for the course with the book: Rodent Models of Stroke

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Course

'Cerebral Ischemia: in vivo and in vitro models'

During the course participants will be exposed to all relevant in vivo or in vitro experimental models in cerebral ischemia and stroke research. A major focus lies on the understanding of the differential validity and limitations of the various models and their outcome parameters. In addition, all models will be discussed in the context of their clinical relevance. 

Programm: Monday, 29.4.2019 'in vivo ischemia'

  • 09:30 - 10:00 The participants "What is my background, and what is my interest?"
  • 10:00 - 12:30 Dirnagl Introduction and overview 'Pathophysiology instructs modelling of cerebral ischemia' (with a break in between...) 
  • 12:30 - 13:30 Lunch break 
  • 13:30-14:30 Khalil  MR of rodent stroke 
  • 14:45-15:45 Bernard Rodent models for stroke 
  • 15:45-16:00 Break 
  • 16:00-17:00 Rex Assessment of outcome I: Behavioral testing after MCAO 
  • 17:00-18:00 Przesdzing Assessment of outcome II: Ex vivo assessment of cell death and damage after MCAO

Programm: Tuesday, 30.4.2019 'in vitro ischemia'

  •  09:00 - 10:15 Dirnagl 'Quality issues in cerebral ischemia research' 
  • 10:15 - 10:30 Break 
  • 10:30 - 12:30 Cerdeira Neuronal cell cultures in in vitro ischemia research: General aspects and quantification of damage-The oxygen glucose deprivation (OGD) model 
  • 12:30 - 13:30 Lunch break 
  • 13:30 - 15:30 Freyer / Rex Cell culture / OGD demonstration und discussion in the lab (half of the participants, 45 min each) In vivo ischemia demonstration und discussion in the lab (half of the participants, 45 min each) 
  • 15:30 - Dirnagl and the participants

Final discussion
Interested participants are offered a tour through the Dept. of Experimental Neurology and/or Department of Neurology